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FMDV (SAT-1) Protease 3C Recombinant Protein (N-His) (VK164042)

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Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

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  • Даташит

  • SDS

Обзор
Каталожный номерVK164042
Описание
FMDV (SAT-1) Protease 3C Recombinant Protein (N-His) (VK164042) expressed in E. coli. Purity: >90% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
  • ●Recombinant — Consistent lot-to-lot performance.
  • ●High Purity — >90% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
Система экспрессииE. coli
Номер доступаA0A7L4XQ62
Длина белкаSer1657-Glu1869
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыFoot-and-mouth disease virus (FMDV strain SAT-1)
Характер Recombinant
Уровень эндотоксинов Remove endotoxin and sterilization is required for cell assay.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 25.03 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияPicornain 3C, Protease 3C
Фон

Genome polyprotein (Protease 3C) is a ~251 kDa protein. Capsid proteins VP1, VP2, and VP3 form a closed capsid enclosing the viral positive strand RNA genome. All these proteins contain a beta-sheet structure called beta-barrel jelly roll. Together they form an icosahedral capsid (T=3) composed of 60 copies of each VP1, VP2, and VP3, with a diameter of approximately 300 Angstroms. VP1 is situated at the 12 fivefold axes, whereas VP2 and VP3 are located at the quasi-sixfold axes. The naked capsid interacts with the host receptor HAVCR1 to provide virion attachment to and probably entry into the target cell.

Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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