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  • 100ug
  • 1mg
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Обзор
Каталожный номерHW632011
Описание
GMP Grade Human IL-13 (Tag Free) (HW632011) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
  • ●Recombinant — Consistent lot-to-lot performance.
  • ●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
  • ●Bioactive — Verified biological activity.
Система экспрессииMammalian cells
Номер доступаP35225
Длина белкаGly35-Asn146
ПримененияELISA, Functional assay
ВидыHomo sapiens (Human)
Биологическая активность Measured in a cell proliferation assay using TF-1 cells. The ED50 ≤1.0ng/ml,corresponding to a specificBioactivity of ≥1×106 U/mg.
Характер Recombinant
Цель IL-13, IL13, Interleukin-13, NC30
Уровень эндотоксинов <0.01 EU/ug
Чистота >95% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 12.3 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияIL-13, IL13, Interleukin-13, NC30
Фон

Interleukin-13 (IL13,NC30,Interleukin-13,IL-13) is a ~15 kDa protein. Cytokine that plays important roles in allergic inflammation and immune response to parasite infection. Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells. Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1. Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF through a mechanism that partially involves suppression of NF-kappa-B.

1. Minty, A. et al. (1993) Nature 362, 248-50. PMID: 8096327
2. McKenzie, AN. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 3735-9. PMID: 8097324
3. Defrance, T. et al. (1994) The Journal of experimental medicine 179, 135-43. PMID: 7903680
4. Luttmann, W. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 1678-83. PMID: 8759755
5. Schnyder, B. et al. (1996) Blood 87, 4286-95. PMID: 8639787
6. Miloux, B. et al. (1997) FEBS letters 401, 163-6. PMID: 9013879
7. Kasaian, MT. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 187, 561-9. PMID: 21622864
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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