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Anti-AAV6 VP1 Polyclonal Antibody (VK130014)

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Vista general
Número de catálogoVK130014
Descripción
Anti-AAV6 VP1/Capsid protein VP1 Polyclonal Antibody (VK130014) is a rabbit polyclonal antibody detecting Capsid protein VP1. Suitable for Adeno-associated virus - 6.
Reactividad de especiesAdeno-associated virus 6 (AAV6)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant AAV6 VP1/Capsid protein VP1 (Ala2-Leu736).
Objetivo Capsid protein VP1
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso O56137
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

Genome polyprotein (VP1/Capsid protein VP1) is a ~246 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host epithelial cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

1. Hogle, JM. et al. (1985) Science (New York, N.Y.) 229, 1358-65. PMID: 2994218
2. Strauss, M. et al. (2013) Journal of virology 87, 3903-14. PMID: 23365424
3. Strauss, M. et al. (2015) Journal of virology 89, 4143-57. PMID: 25631086
4. Brandenburg, B. et al. (2007) PLoS biology 5, e183. PMID: 17622193
5. Coyne, CB. et al. (2007) The EMBO journal 26, 4016-28. PMID: 17717529
6. Bergelson, JM. (2008) Trends in microbiology 16, 44-7. PMID: 18191571
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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