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Обзор
Каталожный номерHF952014
Описание
Anti-ABCB7 Polyclonal Antibody (HF952014) is a rabbit polyclonal antibody detecting ABCB7 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human ABCB7 (Val472-Cys752).
Цель ABC transporter 7 protein, ABC7, ATP-binding cassette sub-family B member 7, mitochondrial, ATP-binding cassette transporter 7, ABCB7
Очистка Purified by antigen affinity column.
Номер доступа O75027
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Iron-sulfur clusters transporter ABCB7, mitochondrial is a ~82 kDa protein. Exports glutathione-coordinated iron-sulfur clusters such as [2Fe-2S]-(GS)4 cluster from the mitochondria to the cytosol in an ATP-dependent manner allowing the assembly of the cytosolic iron-sulfur (Fe/S) cluster-containing proteins and participates in iron homeostasis. Moreover, through a functional complex formed of ABCB7, FECH and ABCB10, also plays a role in the cellular iron homeostasis, mitochondrial function and heme biosynthesis. In cardiomyocytes, regulates cellular iron homeostasis and cellular reactive oxygen species (ROS) levels through its interaction with COX4I1. May also play a role in hematopoiesis.

1. Allikmets, R. et al. (1999) Human molecular genetics 8, 743-9. PMID: 10196363
2. Cavadini, P. et al. (2007) Blood 109, 3552-9. PMID: 17192393
3. Pearson, SA. et al. (2021) Archives of biochemistry and biophysics 697, 108661. PMID: 33157103
4. Maio, N. et al. (2019) Haematologica 104, 1756-1767. PMID: 30765471
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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