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Anti-ABCE1 Polyclonal Antibody (HX969014)

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Aperçu
Numéro de catalogueHX969014
Description
Anti-ABCE1 Polyclonal Antibody (HX969014) is a rabbit polyclonal antibody detecting ABCE1 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ABCE1 (Ile389-Asn556).
Cible HuHP68, RNS4I, RNASEL1, RNase L inhibitor, 2'-5'-oligoadenylate-binding protein, RLI, ABCE1, ATP-binding cassette sub-family E member 1, RNASELI, Ribonuclease 4 inhibitor
Purification Purified by antigen affinity column.
Numéro d'accès P61221
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

ATP-binding cassette sub-family E member 1 (ABCE1) is a ~67 kDa protein. Nucleoside-triphosphatase (NTPase) involved in ribosome recycling by mediating ribosome disassembly. Able to hydrolyze ATP, GTP, UTP and CTP. Splits ribosomes into free 60S subunits and tRNA- and mRNA-bound 40S subunits. Acts either after canonical termination facilitated by release factors (ETF1/eRF1) or after recognition of stalled and vacant ribosomes by mRNA surveillance factors (PELO/Pelota). Involved in the No-Go Decay (NGD) pathway: recruited to stalled ribosomes by the Pelota-HBS1L complex, and drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.

1. Pisarev, AV. et al. (2010) Molecular cell 37, 196-210. PMID: 20122402
2. Pisareva, VP. et al. (2011) The EMBO journal 30, 1804-17. PMID: 21448132
3. Wu, Z. et al. (2018) Cell metabolism 28, 130-144.e7. PMID: 29861391
5. Le Roy, F. et al. (2001) The Journal of biological chemistry 276, 48473-82. PMID: 11585831
Note For research use only.
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-ABCE1 Polyclonal Antibody

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