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Anti-ACAA2 Polyclonal Antibody (HW427014)

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Anti-ACAA2 Polyclonal Antibody
Anti-ACAA2 Polyclonal Antibody
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Vista general
Número de catálogoHW427014
Descripción
Anti-ACAA2 Polyclonal Antibody (HW427014) is a rabbit polyclonal antibody detecting ACAA2 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pongo abelii, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ACAA2 (Arg90-Ala397).
Objetivo Acetyl-CoA acyltransferase, Mitochondrial 3-oxoacyl-CoA thiolase, T1, Beta-ketothiolase, 3-ketoacyl-CoA thiolase, mitochondrial, Acyl-CoA hydrolase, mitochondrial, ACAA2, Acetyl-CoA acetyltransferase
Purificación Purified by antigen affinity column.
Número de acceso P42765
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

3-ketoacyl-CoA thiolase, mitochondrial (ACAA2) is a ~41 kDa protein. In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable).

1. Kiema, TR. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 3212-25. PMID: 25478839
2. Cao, W. et al. (2008) Biochimica et biophysica acta 1780, 873-80. PMID: 18371312
Nota For research use only.
Imágenes
  • Anti-ACAA2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ACAA2 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse heart lysate
    Lane 2: Mouse liver lysate
    Lane 3: Rat heart lysate
    Lane 4: Rat liver lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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