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Anti-ACAT1 Polyclonal Antibody (HB220014)

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Anti-ACAT1 Polyclonal Antibody
Anti-ACAT1 Polyclonal Antibody
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  • 100ug
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Vista general
Número de catálogoHB220014
Descripción
Anti-ACAT1 Polyclonal Antibody (HB220014) is a rabbit polyclonal antibody detecting ACAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ACAT1 (Ala111-Leu427).
Objetivo ACAT, Acetoacetyl-CoA thiolase, MAT, Acetyl-CoA acetyltransferase, mitochondrial, ACAT1, T2
Purificación Purified by antigen affinity column.
Número de acceso P24752
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) is a ~45 kDa protein. This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.

1. Fukao, T. et al. (1991) Biochemical and biophysical research communications 179, 124-9. PMID: 1715688
2. Wakazono, A. et al. (1995) Human mutation 5, 34-42. PMID: 7728148
3. Fukao, T. et al. (1998) Human mutation 12, 245-54. PMID: 9744475
4. Haapalainen, AM. et al. (2007) Biochemistry 46, 4305-21. PMID: 17371050
Nota For research use only.
Imágenes
  • Anti-ACAT1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ACAT1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Caco2 cell lysate
    Lane 2: Mouse heart lysate
    Lane 3: Mouse liver lysate
    Lane 4: Rat heart lysate
    Lane 5: Rat skeletal muscle lysate
    Lane 6: Rat liver lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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