Anti-ACAT1 Polyclonal Antibody (HB220014)

Name: Anti-ACAT1 Polyclonal Antibody
Brand: abinScience
SKU: HB220014
Price: 96 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) : --

Spécification :

20ug
50ug
100ug

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HB220014

Description

Anti-ACAT1 Polyclonal Antibody (HB220014) is a rabbit polyclonal antibody detecting ACAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ACAT1 (Ala111-Leu427).

Cible

ACAT, Acetoacetyl-CoA thiolase, MAT, Acetyl-CoA acetyltransferase, mitochondrial, ACAT1, T2

Purification

Purified by antigen affinity column.

Numéro d'accès

P24752

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) is a ~45 kDa protein. This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.

1. Fukao, T. et al. (1991) Biochemical and biophysical research communications 179, 124-9. PMID: 1715688

2. Wakazono, A. et al. (1995) Human mutation 5, 34-42. PMID: 7728148

3. Fukao, T. et al. (1998) Human mutation 12, 245-54. PMID: 9744475

4. Haapalainen, AM. et al. (2007) Biochemistry 46, 4305-21. PMID: 17371050

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ACAT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Caco2 cell lysate
Lane 2: Mouse heart lysate
Lane 3: Mouse liver lysate
Lane 4: Rat heart lysate
Lane 5: Rat skeletal muscle lysate
Lane 6: Rat liver lysate

Reférences

Fiche technique

Anti-ACAT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-ACAT1 Polyclonal Antibody (HB220014)

Aperçu

Images

Reférences

Fiche technique

SDS