中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HB220014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-ACAT1 Polyclonal Antibody (HB220014) is a rabbit polyclonal antibody detecting ACAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.
Highlights
|
||||||||
| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ACAT1 (Ala111-Leu427). | ||||||||
| ターゲット | ACAT, Acetoacetyl-CoA thiolase, MAT, Acetyl-CoA acetyltransferase, mitochondrial, ACAT1, T2 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P24752 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) is a ~45 kDa protein. This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism. 1. Fukao, T. et al. (1991) Biochemical and biophysical research communications 179, 124-9. PMID: 1715688 2. Wakazono, A. et al. (1995) Human mutation 5, 34-42. PMID: 7728148 3. Fukao, T. et al. (1998) Human mutation 12, 245-54. PMID: 9744475 4. Haapalainen, AM. et al. (2007) Biochemistry 46, 4305-21. PMID: 17371050 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-ACAT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Caco2 cell lysate
Lane 2: Mouse heart lysate
Lane 3: Mouse liver lysate
Lane 4: Rat heart lysate
Lane 5: Rat skeletal muscle lysate
Lane 6: Rat liver lysate
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
