Anti-ACO1 Polyclonal Antibody (HB808014)

Name: Anti-ACO1 Polyclonal Antibody
Brand: abinScience
SKU: HB808014
Price: 96 USD
Availability: InStock

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Spécification :

20ug
50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HB808014

Description

Anti-ACO1 Polyclonal Antibody (HB808014) is a rabbit polyclonal antibody detecting ACO1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human ACO1 (Lys26-Ile243).

Cible

Cytoplasmic aconitate hydratase, Aconitase, EC:4.2.1.3, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, IRP1, Iron-responsive element-binding protein 1, IRE-BP 1, ACO1, IREB1

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P21399, P28271, Q63270

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Cytoplasmic aconitate hydratase (ACO1) is a ~98 kDa protein. Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.

1. Haile, DJ. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 11735-9. PMID: 1281544

2. Kaptain, S. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 10109-13. PMID: 1946430

3. Philpott, CC. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 7321-5. PMID: 8041788

4. Stehling, O. et al. (2013) Cell metabolism 18, 187-98. PMID: 23891004

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-ACO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: U2OS cell lysate
Lane 3: 293T cell lysate
Lane 4: HepG2 cell lysate
Lane 5: A549 cell lysate
Lane 6: Mouse liver lysate
Lane 7: Rat liver lysate

Reférences

Fiche technique

Anti-ACO1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-ACO1 Polyclonal Antibody (HB808014)

Aperçu

Images

Reférences

Fiche technique

SDS