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Anti-ACTB/β-actin/Beta Actin Polyclonal Antibody (HX034014)

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Vista general
Número de catálogoHX034014
Descripción
Anti-ACTB/β-actin/Beta Actin Polyclonal Antibody (HX034014) is a rabbit polyclonal antibody detecting Beta-actin in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human β-actin (Met1-Phe375).
Objetivo Beta-actin, ACTB, Actin, cytoplasmic 1
Purificación Purified by antigen affinity column.
Número de acceso P60709
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Actin, cytoplasmic 1 (ACTB/β-actin) is a ~41 kDa protein. Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built.

1. Hundt, N. et al. (2014) The FEBS journal 281, 5279-91. PMID: 25255767
2. Drazic, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 4399-4404. PMID: 29581253
3. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947
4. Serna, M. et al. (2024) Developmental cell 59, 3175-3188.e8. PMID: 39321809
5. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ACTB/β-actin/Beta Actin Polyclonal Antibody

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