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| カタログ番号 | HX058014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-ACTG1 Polyclonal Antibody (HX058014) is a rabbit polyclonal antibody detecting ACTG1 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Absidia glauca.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ACTG1 (Met1-Phe375). | ||||||||
| ターゲット | ACTG, Gamma-actin, ACTG1, Actin, cytoplasmic 2 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P60710, P60709, P60711, P63260, P63259, P63261, P68136, P68133, P68033, P68032, P68134, P62736, P63269, P68035, P63268, P62738, P63267, P62737, Q8BFZ3, Q6S8J3, Q562R1, P0CG38, P0CG39, A5A3E0, Q9BYX7 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Actin, cytoplasmic 1 (ACTG1) is a ~41 kDa protein. Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built. 1. Baarlink, C. et al. (2013) Science (New York, N.Y.) 340, 864-7. PMID: 23558171 2. Plessner, M. et al. (2015) The Journal of biological chemistry 290, 11209-16. PMID: 25759381 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-ACTG1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human ACTG1 (Catalog No: HX058012)
Lane 2: PC12 cell lysate
Lane 3: MCF7 cell lysate
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