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Anti-ADK Polyclonal Antibody (HX989014)

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Aperçu
Numéro de catalogueHX989014
Description
Anti-ADK Polyclonal Antibody (HX989014) is a rabbit polyclonal antibody detecting ADK in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Cricetulus griseus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ADK (Ile94-Ala339).
Cible Adenosine 5'-phosphotransferase, ADK, AK, Adenosine kinase
Purification Purified by antigen affinity column.
Numéro d'accès P55263
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Adenosine kinase (ADK) is a ~40 kDa protein. Adenosine kinase that mediates the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner: catalyzes phosphorylation of both unmodified and modified adenosines. Plays a key role in the detoxification of modified adenosines containing N(6)-methylated adenine (m6A) post-transcriptional modification. Modified nucleosides are derived from the degradation of RNAs (mRNAs, rRNAs and tRNAs) and possess intrinsic cytotoxicity and must be cleared to prevent metabolic dysfunction. Catalyzes the phosphorylation of the free cytosolic methylated adenosine nucleotides N(6)-methyladenosine (m6A), N(6),N(6)-dimethyladenosine (m6,6A) and N(6)-isopentenyladenosine (i6A) into adenosine monophosphate (AMP) intermediates that are further detoxified by MAPDA/ADAL.

1. Bjursell, MK. et al. (2011) American journal of human genetics 89, 507-15. PMID: 21963049
2. Ogawa, A. et al. (2025) Cell 188, 6151-6169.e24. PMID: 40840445
3. Palella, TD. et al. (1980) The Journal of biological chemistry 255, 5264-9. PMID: 6246102
4. Spychala, J. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 1232-7. PMID: 8577746
5. McNally, T. et al. (1997) Biochemical and biophysical research communications 231, 645-50. PMID: 9070863
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ADK Polyclonal Antibody

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