Anti-ADPRS Polyclonal Antibody (HB443014)

Name: Anti-ADPRS Polyclonal Antibody
Brand: abinScience
SKU: HB443014
Price: 96 USD
Availability: InStock

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Número de catálogo

HB443014

Descripción

Anti-ADPRS Polyclonal Antibody (HB443014) is a rabbit polyclonal antibody detecting ADP-ribosylhydrolase ARH3; ADP-ribose glycohydrolase ARH3; ADP-ribosylhydrolase 3; O-acetyl-ADP-ribose deacetylase ARH3; 3.5.1.-; Poly(ADP-ribose) glycohydrolase ARH3; 3.2.1.143; [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; 3.2.2.-; ADPRS; ADPRHL2; ARH3 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant human ADPRS (Ser19-Ser363).

Objetivo

ADP-ribosylhydrolase ARH3; ADP-ribose glycohydrolase ARH3; ADP-ribosylhydrolase 3; O-acetyl-ADP-ribose deacetylase ARH3; 3.5.1.-; Poly(ADP-ribose) glycohydrolase ARH3; 3.2.1.143; [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; 3.2.2.-; ADPRS; ADPRHL2; ARH3

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

Q9NX46, Q3SYV9, Q8CG72

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

ADP-ribosylhydrolase ARH3 (ADPRS) is a ~38 kDa protein. ADP-ribosylhydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine and threonine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose. Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds. Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos.

1. Kasamatsu, A. et al. (2011) The Journal of biological chemistry 286, 21110-7. PMID: 21498885

2. Pourfarjam, Y. et al. (2018) The Journal of biological chemistry 293, 12350-12359. PMID: 29907568

3. Wang, M. et al. (2018) The Journal of biological chemistry 293, 14470-14480. PMID: 30045870

4. Danhauser, K. et al. (2018) American journal of human genetics 103, 817-825. PMID: 30401461

5. Mashimo, M. et al. (2019) JCI insight 4. PMID: 30830864

6. Bonfiglio, JJ. et al. (2020) Cell 183, 1086-1102.e23. PMID: 33186521

7. Voorneveld, J. et al. (2021) Chemistry (Weinheim an der Bergstrasse, Germany) 27, 10621-10627. PMID: 33769608

8. Pourfarjam, Y. et al. (2021) The Journal of biological chemistry 296, 100692. PMID: 33894202

9. Prokhorova, E. et al. (2021) Molecular cell 81, 2640-2655.e8. PMID: 34019811

10. Rack, JGM. et al. (2021) Nature communications 12, 4581. PMID: 34321462

Nota

For research use only

Imágenes

Western blot

Western blot analysis was performed using anti-ADPRS polyclonal antibody at 1ug/mL on various samples.
Lane 1: Rat brain lysate
Lane 2: Rat heart lysate

Referencias

Datasheet

Anti-ADPRS Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-ADPRS Polyclonal Antibody (HB443014)

Vista general

Imágenes

Referencias

Datasheet

SDS