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Anti-ADRA1A Polyclonal Antibody (HW676014)

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Vista general
Número de catálogoHW676014
Descripción
Anti-ADRA1A Polyclonal Antibody (HW676014) is a rabbit polyclonal antibody detecting ADRA1A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ADRA1A (Ser330-Val466).
Objetivo ADRA1C, ADRA1A, Alpha-1A adrenoreceptor, Alpha-1C adrenergic receptor, Alpha-adrenergic receptor 1c, Alpha-1A adrenoceptor, Alpha-1A adrenergic receptor
Purificación Purified by antigen affinity column.
Número de acceso P35348
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Alpha-1A adrenergic receptor (ADRA1A) is a ~51 kDa protein. Alpha-1 adrenergic receptors are G protein-coupled receptors for catecholamines that signal through the G(q) family of G proteins, including G(q) and G(11). Upon activation, they stimulate the phosphatidylinositol-calcium second messenger pathway, leading to calcium release from intracellular stores and activation of protein kinase C. ADRA1A binds the catecholamine ligands norepinephrine and epinephrine. Can also couple to G(14) protein. Nuclear ADRA1A forms heterooligomers with ADRA1B to regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.

1. Su, M. et al. (2023) Nature communications 14, 4819. PMID: 37563160
2. Wright, CD. et al. (2008) Circulation research 103, 992-1000. PMID: 18802028
3. Schwinn, DA. et al. (1995) The Journal of pharmacology and experimental therapeutics 272, 134-42. PMID: 7815325
4. Weinberg, DH. et al. (1994) Biochemical and biophysical research communications 201, 1296-304. PMID: 8024574
5. Forray, C. et al. (1994) Molecular pharmacology 45, 703-8. PMID: 8183249
6. Shibata, K. et al. (1996) British journal of pharmacology 118, 1403-8. PMID: 8832064
7. Wright, CD. et al. (2012) Cellular signalling 24, 794-802. PMID: 22120526
8. Ogata, T. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 3811-6. PMID: 24567387
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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