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Anti-ADRA1A Polyclonal Antibody (HW676014)

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Aperçu
Numéro de catalogueHW676014
Description
Anti-ADRA1A Polyclonal Antibody (HW676014) is a rabbit polyclonal antibody detecting ADRA1A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ADRA1A (Ser330-Val466).
Cible ADRA1C, ADRA1A, Alpha-1A adrenoreceptor, Alpha-1C adrenergic receptor, Alpha-adrenergic receptor 1c, Alpha-1A adrenoceptor, Alpha-1A adrenergic receptor
Purification Purified by antigen affinity column.
Numéro d'accès P35348
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Alpha-1A adrenergic receptor (ADRA1A) is a ~51 kDa protein. Alpha-1 adrenergic receptors are G protein-coupled receptors for catecholamines that signal through the G(q) family of G proteins, including G(q) and G(11). Upon activation, they stimulate the phosphatidylinositol-calcium second messenger pathway, leading to calcium release from intracellular stores and activation of protein kinase C. ADRA1A binds the catecholamine ligands norepinephrine and epinephrine. Can also couple to G(14) protein. Nuclear ADRA1A forms heterooligomers with ADRA1B to regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.

1. Su, M. et al. (2023) Nature communications 14, 4819. PMID: 37563160
2. Wright, CD. et al. (2008) Circulation research 103, 992-1000. PMID: 18802028
3. Schwinn, DA. et al. (1995) The Journal of pharmacology and experimental therapeutics 272, 134-42. PMID: 7815325
4. Weinberg, DH. et al. (1994) Biochemical and biophysical research communications 201, 1296-304. PMID: 8024574
5. Forray, C. et al. (1994) Molecular pharmacology 45, 703-8. PMID: 8183249
6. Shibata, K. et al. (1996) British journal of pharmacology 118, 1403-8. PMID: 8832064
7. Wright, CD. et al. (2012) Cellular signalling 24, 794-802. PMID: 22120526
8. Ogata, T. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 3811-6. PMID: 24567387
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ADRA1A Polyclonal Antibody

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