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| カタログ番号 | HA744014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-AFG2A Polyclonal Antibody (HA744014) is a rabbit polyclonal antibody detecting ATPase family gene 2 protein homolog A; 3.6.4.10; AFG2 AAA ATPase homolog A; Ribosome biogenesis protein SPATA5; Spermatogenesis-associated factor protein; Spermatogenesis-associated protein 5; AFG2A; SPAF; SPATA5 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant human AFG2A (Thr351-Leu893). | ||||||||
| ターゲット | ATPase family gene 2 protein homolog A; 3.6.4.10; AFG2 AAA ATPase homolog A; Ribosome biogenesis protein SPATA5; Spermatogenesis-associated factor protein; Spermatogenesis-associated protein 5; AFG2A; SPAF; SPATA5 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q8NB90, Q3UMC0 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | ATPase family gene 2 protein homolog A (AFG2A) is a ~97 kDa protein. ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity. Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles. 1. Krishnamoorthy, V. et al. (2024) Cell 187, 2250-2268.e31. PMID: 38554706 2. Ni, C. et al. (2022) Cell reports 38, 110597. PMID: 35354024 | ||||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-AFG2A polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: LO2 cell lysate
Lane 3: Mouse liver lysate
Lane 4: Mouse brain lysate
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