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Anti-AIF1/IBA1 Polyclonal Antibody (HX192014)

Anti-AIF1/IBA1 Polyclonal Antibody
Anti-AIF1/IBA1 Polyclonal Antibody
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Aperçu
Numéro de catalogueHX192014
Description
Anti-AIF1/IBA1 Polyclonal Antibody (HX192014) is a rabbit polyclonal antibody detecting AIF1 in ELISA, IHC, WB, IF. Suitable for Mouse, Bovine, Human, Macaca mulatta, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB, IF
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human AIF1/IBA1 (Met1-Pro147).
Cible Ionized calcium-binding adapter molecule 1, AIF1, AIF-1, Allograft inflammatory factor 1, IBA1, G1, Protein G1
Purification Purified by antigen affinity column.
Numéro d'accès P55008
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Allograft inflammatory factor 1 (AIF1/IBA1) is a ~16 kDa protein. Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function.

Note For research use only.
Images
  • Anti-AIF1/IBA1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AIF1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: THP-1 cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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