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![Human AIM2[1-343] Recombinant Protein (N-His)](/static/images/logo.png){kind=logo}
| カタログ番号 | HT230014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-AIM2 Polyclonal Antibody (HT230014) is a rabbit polyclonal antibody detecting AIM2 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human AIM2 (Met1-Lys342). | ||||||||
| ターゲット | Absent in melanoma 2, Interferon-inducible protein AIM2, AIM2 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O14862 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Interferon-inducible protein AIM2 is a ~38 kDa protein. Sensor component of the AIM2 inflammasome, which mediates inflammasome activation in response to the presence of double-stranded DNA (dsDNA) in the cytosol, leading to subsequent pyroptosis. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. Acts as a recognition receptor (PRR): specifically recognizes and binds dsDNA in the cytosol, and mediates the formation of the inflammasome polymeric complex composed of AIM2, CASP1 and PYCARD/ASC. Recruitment of pro-caspase-1 (proCASP1) to the AIM2 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion. In some cells, CASP1 activation mediates cleavage and activation of GSDMD, triggering pyroptosis without promoting cytokine secretion. 1. Woerner, SM. et al. (2007) Genes, chromosomes & cancer 46, 1080-9. PMID: 17726700 2. Hornung, V. et al. (2009) Nature 458, 514-8. PMID: 19158675 3. Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13. PMID: 19158676 4. Bürckstümmer, T. et al. (2009) Nature immunology 10, 266-72. PMID: 19158679 5. Tsuchiya, K. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 1186-95. PMID: 20566831 6. Jin, T. et al. (2013) The Journal of biological chemistry 288, 13225-35. PMID: 23530044 7. Morrone, SR. et al. (2015) Nature communications 6, 7827. PMID: 26197926 8. Lu, A. et al. (2015) Cell discovery 1, 15013-. PMID: 26583071 9. Matyszewski, M. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1963-E1972. PMID: 29440442 10. Matyszewski, M. et al. (2021) Nature communications 12, 2735. PMID: 33980849 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-AIM2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: Jurkat cell lysate
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