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Anti-AK3 Polyclonal Antibody (HV747014)

Anti-AK3 Polyclonal Antibody
Anti-AK3 Polyclonal Antibody
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Aperçu
Numéro de catalogueHV747014
Description
Anti-AK3 Polyclonal Antibody (HV747014) is a rabbit polyclonal antibody detecting AK3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human AK3 (Leu8-Pro227).
Cible GTP:AMP phosphotransferase AK3, mitochondrial, AK 3, AK6, Adenylate kinase 3 alpha-like 1, AK3, AKL3L, AK3L1, Adenylate kinase 3
Purification Purified by antigen affinity column.
Numéro d'accès Q9UIJ7
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

GTP:AMP phosphotransferase AK3, mitochondrial is a ~25 kDa protein. Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity. Could also use ITP as phosphate donor. Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable).

1. Noma, T. et al. (2001) The Biochemical journal 358, 225-32. PMID: 11485571
2. Rogne, P. et al. (2020) Biochemistry 59, 3570-3581. PMID: 32822537
Note For research use only.
Images
  • Anti-AK3 Polyclonal Antibody

    Western blot

    Lane 1: HepG2 cell lysate
    Lane 2: A549 cell lysate
    Lane 3: BxPC-3 cell lysate
    Lane 4: Mouse heart lysate
    Lane 5: Mouse liver lysate
    Lane 6: Mouse kidney lysate
    Lane 7: Rat heart lysate
    Lane 8: Rat liver lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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