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Anti-AK3 Polyclonal Antibody (HV747014)

Anti-AK3 Polyclonal Antibody
Anti-AK3 Polyclonal Antibody
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  • 概要

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概要
カタログ番号HV747014
説明
Anti-AK3 Polyclonal Antibody (HV747014) is a rabbit polyclonal antibody detecting AK3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human, Mouse
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human AK3 (Leu8-Pro227).
ターゲット GTP:AMP phosphotransferase AK3, mitochondrial, AK 3, AK6, Adenylate kinase 3 alpha-like 1, AK3, AKL3L, AK3L1, Adenylate kinase 3
精製 Purified by antigen affinity column.
アクセッション番号 Q9UIJ7
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

GTP:AMP phosphotransferase AK3, mitochondrial is a ~25 kDa protein. Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity. Could also use ITP as phosphate donor. Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable).

1. Noma, T. et al. (2001) The Biochemical journal 358, 225-32. PMID: 11485571
2. Rogne, P. et al. (2020) Biochemistry 59, 3570-3581. PMID: 32822537
注意事項 For research use only.
イメージ
  • Anti-AK3 Polyclonal Antibody

    Western blot

    Lane 1: HepG2 cell lysate
    Lane 2: A549 cell lysate
    Lane 3: BxPC-3 cell lysate
    Lane 4: Mouse heart lysate
    Lane 5: Mouse liver lysate
    Lane 6: Mouse kidney lysate
    Lane 7: Rat heart lysate
    Lane 8: Rat liver lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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