Anti-AKT1 Polyclonal Antibody (HB610014)

Name: Anti-AKT1 Polyclonal Antibody
Brand: abinScience
SKU: HB610014
Price: 168 USD
Availability: InStock

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Fiche technique
SDS

Aperçu

Numéro de catalogue

HB610014

Description

Anti-AKT1 Polyclonal Antibody (HB610014) is a rabbit polyclonal antibody detecting AKT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human AKT1 (Met1-Ala480).

Cible

RAC-alpha serine/threonine-protein kinase, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene c-Akt, Protein kinase B alpha, Protein kinase B, AKT1, PKB

Purification

Purified by antigen affinity column.

Numéro d'accès

P31749

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

RAC-alpha serine/threonine-protein kinase (AKT1) is a ~55 kDa protein. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.

1. Nicholson, KM. et al. (2002) Cellular signalling 14, 381-95. PMID: 11882383

2. Rönnstrand, L. (2004) Cellular and molecular life sciences : CMLS 61, 2535-48. PMID: 15526160

3. Cartlidge, RA. et al. (2005) The EMBO journal 24, 1696-705. PMID: 15861136

4. Heron-Milhavet, L. et al. (2011) Histology and histopathology 26, 651-62. PMID: 21432781

5. Hers, I. et al. (2011) Cellular signalling 23, 1515-27. PMID: 21620960

6. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173

7. Choppara, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 998-1003. PMID: 29343641

Note

For research use only.

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