Anti-AKT1 Polyclonal Antibody (HB610014)

Name: Anti-AKT1 Polyclonal Antibody
Brand: abinScience
SKU: HB610014
Price: 168 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HB610014

説明

Anti-AKT1 Polyclonal Antibody (HB610014) is a rabbit polyclonal antibody detecting AKT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human AKT1 (Met1-Ala480).

ターゲット

RAC-alpha serine/threonine-protein kinase, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene c-Akt, Protein kinase B alpha, Protein kinase B, AKT1, PKB

精製

Purified by antigen affinity column.

アクセッション番号

P31749

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

RAC-alpha serine/threonine-protein kinase (AKT1) is a ~55 kDa protein. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.

1. Nicholson, KM. et al. (2002) Cellular signalling 14, 381-95. PMID: 11882383

2. Rönnstrand, L. (2004) Cellular and molecular life sciences : CMLS 61, 2535-48. PMID: 15526160

3. Cartlidge, RA. et al. (2005) The EMBO journal 24, 1696-705. PMID: 15861136

4. Heron-Milhavet, L. et al. (2011) Histology and histopathology 26, 651-62. PMID: 21432781

5. Hers, I. et al. (2011) Cellular signalling 23, 1515-27. PMID: 21620960

6. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173

7. Choppara, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 998-1003. PMID: 29343641

注意事項

For research use only.

イメージ

参考文献