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| カタログ番号 | HY519014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ALDOC (Asp34-Ser281). | ||||||||
| ターゲット | Brain-type aldolase, ALDOC, ALDC, Fructose-bisphosphate aldolase C | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P09972 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Fructose-bisphosphate aldolase C (ALDOC) is a ~39 kDa protein. Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis. | ||||||||
| 注意事項 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALDOC (HY519014) at a concentration of 24 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALDOC was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-ALDOC polyclonal antibody at 1μg/ml on various samples.
Lane 1: MCF7 cell lysate
Lane 2: LO2 cell lysate
Lane 3: k562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Mouse brain lysate
Lane 6: Mouse kidney lysate
Lane 7: Mouse muscle lysate
Lane 8: Mouse heart lysate
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