Anti-ANG Polyclonal Antibody (HY052014)

Name: Anti-ANG Polyclonal Antibody
Brand: abinScience
SKU: HY052014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Datasheet
SDS

Vista general

Número de catálogo

HY052014

Descripción

Anti-ANG Polyclonal Antibody (HY052014) is a rabbit polyclonal antibody detecting ANG in ELISA, IHC, WB. Suitable for Human, Pan troglodytes, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human ANG (Gln25-Pro147).

Objetivo

Ribonuclease 5, Angiogenin, RNase 5, RNASE5, ANG

Purificación

Purified by antigen affinity column.

Número de acceso

P03950

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Angiogenin is a ~16 kDa protein. Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context. Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus. Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation. tiRNas also prevent formation of apoptosome, thereby promoting cell survival. Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36.

1. Xu, ZP. et al. (2002) Biochemical and biophysical research communications 294, 287-92. PMID: 12051708

2. Saxena, SK. et al. (1992) The Journal of biological chemistry 267, 21982-6. PMID: 1400510

3. Yamasaki, S. et al. (2009) The Journal of cell biology 185, 35-42. PMID: 19332886

4. Emara, MM. et al. (2010) The Journal of biological chemistry 285, 10959-68. PMID: 20129916

5. Ivanov, P. et al. (2011) Molecular cell 43, 613-23. PMID: 21855800

6. Thiyagarajan, N. et al. (2012) Nature communications 3, 1121. PMID: 23047679

7. Pizzo, E. et al. (2013) Journal of cell science 126, 4308-19. PMID: 23843625

8. Shapiro, R. et al. (1986) Biochemistry 25, 3527-32. PMID: 2424496

9. Harper, JW. et al. (1988) Proceedings of the National Academy of Sciences of the United States of America 85, 7139-43. PMID: 2459697

10. Lee, FS. et al. (1989) Biochemical and biophysical research communications 161, 121-6. PMID: 2730651

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-ANG Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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