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| カタログ番号 | HY052014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-ANG Polyclonal Antibody (HY052014) is a rabbit polyclonal antibody detecting ANG in ELISA, IHC, WB. Suitable for Human, Pan troglodytes, and Pongo pygmaeus.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ANG (Gln25-Pro147). | ||||||||
| ターゲット | Ribonuclease 5, Angiogenin, RNase 5, RNASE5, ANG | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P03950 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Angiogenin is a ~16 kDa protein. Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context. Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus. Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation. tiRNas also prevent formation of apoptosome, thereby promoting cell survival. Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36. 1. Xu, ZP. et al. (2002) Biochemical and biophysical research communications 294, 287-92. PMID: 12051708 2. Saxena, SK. et al. (1992) The Journal of biological chemistry 267, 21982-6. PMID: 1400510 3. Yamasaki, S. et al. (2009) The Journal of cell biology 185, 35-42. PMID: 19332886 4. Emara, MM. et al. (2010) The Journal of biological chemistry 285, 10959-68. PMID: 20129916 5. Ivanov, P. et al. (2011) Molecular cell 43, 613-23. PMID: 21855800 6. Thiyagarajan, N. et al. (2012) Nature communications 3, 1121. PMID: 23047679 7. Pizzo, E. et al. (2013) Journal of cell science 126, 4308-19. PMID: 23843625 8. Shapiro, R. et al. (1986) Biochemistry 25, 3527-32. PMID: 2424496 9. Harper, JW. et al. (1988) Proceedings of the National Academy of Sciences of the United States of America 85, 7139-43. PMID: 2459697 10. Lee, FS. et al. (1989) Biochemical and biophysical research communications 161, 121-6. PMID: 2730651 | ||||||||
| 注意事項 | For research use only. |
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