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Anti-AOAH Polyclonal Antibody (HB957014)

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Anti-AOAH Polyclonal Antibody
Anti-AOAH Polyclonal Antibody
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Vista general
Número de catálogoHB957014
Descripción
Anti-AOAH Polyclonal Antibody (HB957014) is a rabbit polyclonal antibody detecting AOAH in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human AOAH (Ser24-Gln300).
Objetivo AOAH, Acyloxyacyl hydrolase
Purificación Purified by antigen affinity column.
Número de acceso P28039
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Acyloxyacyl hydrolase (AOAH) is a ~65 kDa protein. Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.

1. Hagen, FS. et al. (1991) Biochemistry 30, 8415-23. PMID: 1883828
2. Gorelik, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E896-E905. PMID: 29343645
3. Staab, JF. et al. (1994) The Journal of biological chemistry 269, 23736-42. PMID: 8089145
Nota For research use only.
Imágenes
  • Anti-AOAH Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AOAH polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HL-60 cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: HUVEC cell lysate
    Lane 5: BxPC-3 cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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