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Anti-AOAH Polyclonal Antibody (HB957014)

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Anti-AOAH Polyclonal Antibody
Anti-AOAH Polyclonal Antibody
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Aperçu
Numéro de catalogueHB957014
Description
Anti-AOAH Polyclonal Antibody (HB957014) is a rabbit polyclonal antibody detecting AOAH in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human AOAH (Ser24-Gln300).
Cible AOAH, Acyloxyacyl hydrolase
Purification Purified by antigen affinity column.
Numéro d'accès P28039
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Acyloxyacyl hydrolase (AOAH) is a ~65 kDa protein. Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.

1. Hagen, FS. et al. (1991) Biochemistry 30, 8415-23. PMID: 1883828
2. Gorelik, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E896-E905. PMID: 29343645
3. Staab, JF. et al. (1994) The Journal of biological chemistry 269, 23736-42. PMID: 8089145
Note For research use only.
Images
  • Anti-AOAH Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AOAH polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HL-60 cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: HUVEC cell lysate
    Lane 5: BxPC-3 cell lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-AOAH Polyclonal Antibody

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