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Anti-AOAH Polyclonal Antibody
Anti-AOAH Polyclonal Antibody
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Спецификация:
  • 20ug
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHB957014
Описание
Anti-AOAH Polyclonal Antibody (HB957014) is a rabbit polyclonal antibody detecting AOAH in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human AOAH (Ser24-Gln300).
Цель AOAH, Acyloxyacyl hydrolase
Очистка Purified by antigen affinity column.
Номер доступа P28039
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Acyloxyacyl hydrolase (AOAH) is a ~65 kDa protein. Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.

1. Hagen, FS. et al. (1991) Biochemistry 30, 8415-23. PMID: 1883828
2. Gorelik, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E896-E905. PMID: 29343645
3. Staab, JF. et al. (1994) The Journal of biological chemistry 269, 23736-42. PMID: 8089145
Примечание For research use only.
Изображения
  • Anti-AOAH Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AOAH polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HL-60 cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: HUVEC cell lysate
    Lane 5: BxPC-3 cell lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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