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Anti Arabidopsis HSP70 Polyclonal Antibody (PR002014)

Anti Arabidopsis HSP70 Polyclonal Antibody
Anti Arabidopsis HSP70 Polyclonal Antibody
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Aperçu
Numéro de cataloguePR002014
Description
Anti Arabidopsis HSP70 Polyclonal Antibody (PR002014) is a rabbit polyclonal antibody detecting HSP70 in WB, IHC, ELISA. Suitable for Glycine max, Petunia hybrida, Spinacia oleracea, and Zea mays.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesArabidopsis thaliana (Mouse-ear cress), Glycine max (Soybean) (Glycine hispida), Solanum lycopersicum (Tomato)
ApplicationsWB, IHC, ELISA
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Arabidopsis HSP70-3 (Met1-Asn391).
Cible HSP70
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès O65719
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsHeat shock 70 kDa protein 3, AtHsp70-3, Heat shock protein 70-3, Heat shock cognate 70 kDa protein 3, AtHsc70-3, Heat shock cognate protein 70-3, HSP70-3
Contexte

Heat shock 70 kDa protein 3 (HSP70) is a ~71 kDa protein. In cooperation with other chaperones, Hsp70s are key components that facilitate folding of de novo synthesized proteins, assist translocation of precursor proteins into organelles, and are responsible for degradation of damaged protein under stress conditions.

Note For research use only.
Images
  • Anti Arabidopsis HSP70 Polyclonal Antibody

    Western blot

    Lane 1: Hela cell lysate
    Lane 2: A431 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: K562 cell lysate
    Lane 6: NIH3T3 cell lysate
    Lane 7: Mouse brain lysate
    Lane 8: Rat brain lysate
    Lane 9: Rat liver lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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