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Anti Arabidopsis HSP70 Polyclonal Antibody (PR002014)

Anti Arabidopsis HSP70 Polyclonal Antibody
Anti Arabidopsis HSP70 Polyclonal Antibody
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概要
カタログ番号PR002014
説明
Anti Arabidopsis HSP70 Polyclonal Antibody (PR002014) is a rabbit polyclonal antibody detecting HSP70 in WB, IHC, ELISA. Suitable for Glycine max, Petunia hybrida, Spinacia oleracea, and Zea mays.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Arabidopsis thaliana (Mouse-ear cress), Glycine max (Soybean) (Glycine hispida), Solanum lycopersicum (Tomato)
アプリケーションWB, IHC, ELISA
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Arabidopsis HSP70-3 (Met1-Asn391).
ターゲット HSP70
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
精製 Purified by antigen affinity column.
アクセッション番号 O65719
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名Heat shock 70 kDa protein 3, AtHsp70-3, Heat shock protein 70-3, Heat shock cognate 70 kDa protein 3, AtHsc70-3, Heat shock cognate protein 70-3, HSP70-3
背景

Heat shock 70 kDa protein 3 (HSP70) is a ~71 kDa protein. In cooperation with other chaperones, Hsp70s are key components that facilitate folding of de novo synthesized proteins, assist translocation of precursor proteins into organelles, and are responsible for degradation of damaged protein under stress conditions.

注意事項 For research use only.
イメージ
  • Anti Arabidopsis HSP70 Polyclonal Antibody

    Western blot

    Lane 1: Hela cell lysate
    Lane 2: A431 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: K562 cell lysate
    Lane 6: NIH3T3 cell lysate
    Lane 7: Mouse brain lysate
    Lane 8: Rat brain lysate
    Lane 9: Rat liver lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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