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| カタログ番号 | HC270014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-ASAH1 Polyclonal Antibody (HC270014) is a rabbit polyclonal antibody detecting ASAH1 in ELISA, IHC, WB. Suitable for Human, Bovine, Macaca fascicularis, and Pan troglodytes.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ASAH1 (Gln22-Trp395). | ||||||||
| ターゲット | PHP32, ASAH1, ACDase, AC, N-acylethanolamine hydrolase ASAH1, Acid CDase, Acylsphingosine deacylase, N-acylsphingosine amidohydrolase, Putative 32 kDa heart protein, ASAH, Acid ceramidase | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q13510 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Acid ceramidase (ASAH1) is a ~44 kDa protein. Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Has a higher catalytic efficiency towards C12-ceramides versus other ceramides. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used. 1. Li, CM. et al. (1999) Genomics 62, 223-31. PMID: 10610716 2. Ferlinz, K. et al. (2001) The Journal of biological chemistry 276, 35352-60. PMID: 11451951 3. Tsuboi, K. et al. (2005) The Journal of biological chemistry 280, 11082-92. PMID: 15655246 4. Ferraz, MJ. et al. (2016) FEBS letters 590, 716-25. PMID: 26898341 5. Kumar, M. et al. (2023) Human molecular genetics 32, 1888-1900. PMID: 36752535 6. Bernardo, K. et al. (1995) The Journal of biological chemistry 270, 11098-102. PMID: 7744740 7. Yamaguchi, Y. et al. (1994) Journal of biochemistry 116, 704-10. PMID: 7852294 | ||||||||
| 注意事項 | For research use only. |
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