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Anti-ASFV NP419L Polyclonal Antibody (VK110024)

Anti-ASFV NP419L Polyclonal Antibody
Anti-ASFV NP419L Polyclonal Antibody
Precio(USD):
Especificación:
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoVK110024
Descripción
Anti-ASFV NP419L Polyclonal Antibody (VK110024) is a rabbit polyclonal antibody detecting ASFV NP419L in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesAfrican swine fever virus (ASFV)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant ASFV NP419L (Met1-Ile419).
Objetivo DNA ligase, EC:6.5.1.1, Polydeoxyribonucleotide synthase [ATP], LIG
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P35970, P0C990, P0C991, P26813, P0C992
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

DNA ligase (NP419L) is a ~48 kDa protein. Very low-fidelity DNA ligase that seals nicks in double-stranded DNA during DNA repair. Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease. It is not essential for viral replication and recombination. Displays a very low adenylation activity towards DNA with 3'-dideoxy- or 3'-amino-terminated nicks compared to regular nick DNA.

1. Lamarche, BJ. et al. (2005) Biochemistry 44, 8408-17. PMID: 15938630
Nota For research use only
Imágenes
  • Anti-ASFV NP419L Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LIG polyclonal antibody at 1ug/mL on various samples.
    Lane 1: ATG21816 100ng

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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