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Anti-ASFV NP419L Polyclonal Antibody (VK110024)

Anti-ASFV NP419L Polyclonal Antibody
Anti-ASFV NP419L Polyclonal Antibody
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  • 50ug
  • 100ug
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Aperçu
Numéro de catalogueVK110024
Description
Anti-ASFV NP419L Polyclonal Antibody (VK110024) is a rabbit polyclonal antibody detecting ASFV NP419L in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesAfrican swine fever virus (ASFV)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant ASFV NP419L (Met1-Ile419).
Cible DNA ligase, EC:6.5.1.1, Polydeoxyribonucleotide synthase [ATP], LIG
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P35970, P0C990, P0C991, P26813, P0C992
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

DNA ligase (NP419L) is a ~48 kDa protein. Very low-fidelity DNA ligase that seals nicks in double-stranded DNA during DNA repair. Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease. It is not essential for viral replication and recombination. Displays a very low adenylation activity towards DNA with 3'-dideoxy- or 3'-amino-terminated nicks compared to regular nick DNA.

1. Lamarche, BJ. et al. (2005) Biochemistry 44, 8408-17. PMID: 15938630
Note For research use only
Images
  • Anti-ASFV NP419L Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LIG polyclonal antibody at 1ug/mL on various samples.
    Lane 1: ATG21816 100ng

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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