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Anti-ASFV NP419L Polyclonal Antibody
Anti-ASFV NP419L Polyclonal Antibody
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерVK110024
Описание
Anti-ASFV NP419L Polyclonal Antibody (VK110024) is a rabbit polyclonal antibody detecting ASFV NP419L in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовAfrican swine fever virus (ASFV)
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant ASFV NP419L (Met1-Ile419).
Цель DNA ligase, EC:6.5.1.1, Polydeoxyribonucleotide synthase [ATP], LIG
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа P35970, P0C990, P0C991, P26813, P0C992
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

DNA ligase (NP419L) is a ~48 kDa protein. Very low-fidelity DNA ligase that seals nicks in double-stranded DNA during DNA repair. Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease. It is not essential for viral replication and recombination. Displays a very low adenylation activity towards DNA with 3'-dideoxy- or 3'-amino-terminated nicks compared to regular nick DNA.

1. Lamarche, BJ. et al. (2005) Biochemistry 44, 8408-17. PMID: 15938630
Примечание For research use only
Изображения
  • Anti-ASFV NP419L Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LIG polyclonal antibody at 1ug/mL on various samples.
    Lane 1: ATG21816 100ng

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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