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Anti-ASFV UBC/pI215L Polyclonal Antibody (VK475014)

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Número de catálogoVK475014
Descripción
Anti-ASFV UBC/pI215L Polyclonal Antibody (VK475014) is a rabbit polyclonal antibody detecting pI215L in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesAfrican swine fever virus (ASFV)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant ASFV UBC/pI215L (Met1-Thr200).
Objetivo Ubiquitin-conjugating enzyme E2, 2.3.2.23, E2 ubiquitin-conjugating enzyme, UBCv1, Ubiquitin carrier protein, Ubiquitin-protein ligase, pI215L, UBC, BA71V-144, I215L
Purificación Purified by antigen affinity column.
Número de acceso P27949
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Ubiquitin-conjugating enzyme E2 (UBC/pI215L) is a ~24 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome. By controlling the ubiquitination status of specific host proteins, the virus may target them to degradation and thereby optimize the viral replication. May be implicated in the shutoff of protein synthesis through its interactions with components of the host translation machinery. Blocks host RELA nuclear translocation upon cytokine stimulation, thereby impairing NF-kappa-B signaling.

1. Freitas, FB. et al. (2018) Scientific reports 8, 3471. PMID: 29472632
2. Barrado-Gil, L. et al. (2020) Frontiers in microbiology 11, 622907. PMID: 33384682
3. Barrado-Gil, L. et al. (2021) Viruses 13. PMID: 34204411
4. Huang, L. et al. (2021) Journal of immunology (Baltimore, Md. : 1950) 207, 2754-2769. PMID: 34759016
5. Hingamp, PM. et al. (1995) Journal of virology 69, 1785-93. PMID: 7853518
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-ASFV UBC/pI215L Polyclonal Antibody

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