Anti-ASFV UBC/pI215L Polyclonal Antibody (VK475014)

Name: Anti-ASFV UBC/pI215L Polyclonal Antibody
Brand: abinScience
SKU: VK475014
Price: 168 USD
Availability: InStock

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SDS

Обзор

Каталожный номер

VK475014

Описание

Anti-ASFV UBC/pI215L Polyclonal Antibody (VK475014) is a rabbit polyclonal antibody detecting pI215L in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

African swine fever virus (ASFV)

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant ASFV UBC/pI215L (Met1-Thr200).

Цель

Ubiquitin-conjugating enzyme E2, 2.3.2.23, E2 ubiquitin-conjugating enzyme, UBCv1, Ubiquitin carrier protein, Ubiquitin-protein ligase, pI215L, UBC, BA71V-144, I215L

Очистка

Purified by antigen affinity column.

Номер доступа

P27949

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Ubiquitin-conjugating enzyme E2 (UBC/pI215L) is a ~24 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome. By controlling the ubiquitination status of specific host proteins, the virus may target them to degradation and thereby optimize the viral replication. May be implicated in the shutoff of protein synthesis through its interactions with components of the host translation machinery. Blocks host RELA nuclear translocation upon cytokine stimulation, thereby impairing NF-kappa-B signaling.

1. Freitas, FB. et al. (2018) Scientific reports 8, 3471. PMID: 29472632

2. Barrado-Gil, L. et al. (2020) Frontiers in microbiology 11, 622907. PMID: 33384682

3. Barrado-Gil, L. et al. (2021) Viruses 13. PMID: 34204411

4. Huang, L. et al. (2021) Journal of immunology (Baltimore, Md. : 1950) 207, 2754-2769. PMID: 34759016

5. Hingamp, PM. et al. (1995) Journal of virology 69, 1785-93. PMID: 7853518

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-ASFV UBC/pI215L Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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