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| カタログ番号 | HB598014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-ATG4B Polyclonal Antibody (HB598014) is a rabbit polyclonal antibody detecting Cysteine protease ATG4B; 3.4.22.-; AUT-like 1 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 1; Autophagin-1; Autophagy-related protein 4 homolog B; HsAPG4B; hAPG4B; ATG4B; APG4B; AUTL1; KIAA0943 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, and Bovine.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant human ATG4B (Met1-Leu393). | ||||||||
| ターゲット | Cysteine protease ATG4B; 3.4.22.-; AUT-like 1 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 1; Autophagin-1; Autophagy-related protein 4 homolog B; HsAPG4B; hAPG4B; ATG4B; APG4B; AUTL1; KIAA0943 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9Y4P1, A0A0G2QC33, Q8BGE6, Q6PZ03 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Cysteine protease ATG4B is a ~44 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3. 1. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837 2. Tanida, I. et al. (2004) The Journal of biological chemistry 279, 36268-76. PMID: 15187094 3. Scherz-Shouval, R. et al. (2007) The EMBO journal 26, 1749-60. PMID: 17347651 4. Satoo, K. et al. (2009) The EMBO journal 28, 1341-50. PMID: 19322194 5. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865 6. Li, M. et al. (2012) Autophagy 8, 401-12. PMID: 22302004 7. Yang, Z. et al. (2015) The Journal of biological chemistry 290, 26549-61. PMID: 26378241 8. Jo, YK. et al. (2016) Oncotarget 7, 57186-57196. PMID: 27527864 9. Li, Y. et al. (2017) Autophagy 13, 1145-1160. PMID: 28633005 10. Pengo, N. et al. (2017) Nature communications 8, 294. PMID: 28821708 | ||||||||
| 注意事項 | For research use only |
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