Anti-ATG4B Polyclonal Antibody (HB598014)

Name: Anti-ATG4B Polyclonal Antibody
Brand: abinScience
SKU: HB598014
Price: 168 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HB598014

Описание

Anti-ATG4B Polyclonal Antibody (HB598014) is a rabbit polyclonal antibody detecting Cysteine protease ATG4B; 3.4.22.-; AUT-like 1 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 1; Autophagin-1; Autophagy-related protein 4 homolog B; HsAPG4B; hAPG4B; ATG4B; APG4B; AUTL1; KIAA0943 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant human ATG4B (Met1-Leu393).

Цель

Cysteine protease ATG4B; 3.4.22.-; AUT-like 1 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 1; Autophagin-1; Autophagy-related protein 4 homolog B; HsAPG4B; hAPG4B; ATG4B; APG4B; AUTL1; KIAA0943

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

Q9Y4P1, A0A0G2QC33, Q8BGE6, Q6PZ03

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Cysteine protease ATG4B is a ~44 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3.

1. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837

2. Tanida, I. et al. (2004) The Journal of biological chemistry 279, 36268-76. PMID: 15187094

3. Scherz-Shouval, R. et al. (2007) The EMBO journal 26, 1749-60. PMID: 17347651

4. Satoo, K. et al. (2009) The EMBO journal 28, 1341-50. PMID: 19322194

5. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865

6. Li, M. et al. (2012) Autophagy 8, 401-12. PMID: 22302004

7. Yang, Z. et al. (2015) The Journal of biological chemistry 290, 26549-61. PMID: 26378241

8. Jo, YK. et al. (2016) Oncotarget 7, 57186-57196. PMID: 27527864

9. Li, Y. et al. (2017) Autophagy 13, 1145-1160. PMID: 28633005

10. Pengo, N. et al. (2017) Nature communications 8, 294. PMID: 28821708

Примечание

For research use only

Изображения

Ссылки

Даташит

Anti-ATG4B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа