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Anti-ATP2A2 Polyclonal Antibody (HB179014)

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Vista general
Número de catálogoHB179014
Descripción
Anti-ATP2A2 Polyclonal Antibody (HB179014) is a rabbit polyclonal antibody detecting ATP2A2 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Cat, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ATP2A2 (Ile315-Met756).
Objetivo SERCA2, Calcium pump 2, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, ATP2B, SR Ca(2+)-ATPase 2, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, ATP2A2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Purificación Purified by antigen affinity column.
Número de acceso P16615
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) is a ~114 kDa protein. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. Also modulates ER contacts with lipid droplets, mitochondria and endosomes. In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis.

1. Dhitavat, J. et al. (2003) The Journal of investigative dermatology 120, 229-32. PMID: 12542527
2. Dally, S. et al. (2006) The Biochemical journal 395, 249-58. PMID: 16402920
3. Zhao, YG. et al. (2017) Molecular cell 67, 974-989.e6. PMID: 28890335
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ATP2A2 Polyclonal Antibody

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