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Обзор
Каталожный номерHB179014
Описание
Anti-ATP2A2 Polyclonal Antibody (HB179014) is a rabbit polyclonal antibody detecting ATP2A2 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Cat, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human ATP2A2 (Ile315-Met756).
Цель SERCA2, Calcium pump 2, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, ATP2B, SR Ca(2+)-ATPase 2, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, ATP2A2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Очистка Purified by antigen affinity column.
Номер доступа P16615
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) is a ~114 kDa protein. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. Also modulates ER contacts with lipid droplets, mitochondria and endosomes. In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis.

1. Dhitavat, J. et al. (2003) The Journal of investigative dermatology 120, 229-32. PMID: 12542527
2. Dally, S. et al. (2006) The Biochemical journal 395, 249-58. PMID: 16402920
3. Zhao, YG. et al. (2017) Molecular cell 67, 974-989.e6. PMID: 28890335
Примечание For research use only.
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Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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