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Anti-ATP5F1E Polyclonal Antibody (HB493014)

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概要
カタログ番号HB493014
説明
Anti-ATP5F1E Polyclonal Antibody (HB493014) is a rabbit polyclonal antibody detecting mitochondrial; ATPase subunit epsilon; ATP synthase F1 subunit epsilon; ATP5F1E; ATP5E in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant human ATP5F1E (Val2-Glu51).
ターゲット ATP synthase subunit epsilon, mitochondrial; ATPase subunit epsilon; ATP synthase F1 subunit epsilon; ATP5F1E; ATP5E
エンドトキシンレベル Please contact with the lab for this information.
精製 Purified by antigen affinity column.
アクセッション番号 P56381, P05632, Q5VTU8, P56382, P29418
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

ATP synthase F(1) complex subunit epsilon, mitochondrial (ATP5F1E) is a ~5 kDa protein. Subunit epsilon, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256
2. Havlícková, V. et al. (2010) Biochimica et biophysica acta 1797, 1124-9. PMID: 20026007
注意事項 For research use only
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