Anti-ATP5F1E Polyclonal Antibody (HB493014)

Name: Anti-ATP5F1E Polyclonal Antibody
Brand: abinScience
SKU: HB493014
Price: 168 USD
Availability: InStock

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Спецификация:

50ug
100ug

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Каталожный номер

HB493014

Описание

Anti-ATP5F1E Polyclonal Antibody (HB493014) is a rabbit polyclonal antibody detecting mitochondrial; ATPase subunit epsilon; ATP synthase F1 subunit epsilon; ATP5F1E; ATP5E in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant human ATP5F1E (Val2-Glu51).

Цель

ATP synthase subunit epsilon, mitochondrial; ATPase subunit epsilon; ATP synthase F1 subunit epsilon; ATP5F1E; ATP5E

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

P56381, P05632, Q5VTU8, P56382, P29418

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

ATP synthase F(1) complex subunit epsilon, mitochondrial (ATP5F1E) is a ~5 kDa protein. Subunit epsilon, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256

2. Havlícková, V. et al. (2010) Biochimica et biophysica acta 1797, 1124-9. PMID: 20026007

Примечание

For research use only

Изображения

Ссылки

Даташит

Anti-ATP5F1E Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-ATP5F1E Polyclonal Antibody (HB493014)

Обзор

Изображения

Ссылки

Даташит

SDS