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Anti-B2M Polyclonal Antibody
Anti-B2M Polyclonal Antibody
Цена(USD):
Спецификация:
  • 20ug
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHX250014
Описание
Anti-B2M Polyclonal Antibody (HX250014) is a rabbit polyclonal antibody detecting B2M in ELISA, IHC, WB. Suitable for Human, Alouatta seniculus, Aotus azarae, Aotus lemurinus, and Aotus nancymaae.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human B2M (Gln22-Met119).
Цель Beta-2-microglobulin, B2M
Очистка Purified by antigen affinity column.
Номер доступа P61769
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Beta-2-microglobulin (B2M/β2-MG) is a ~13 kDa protein. Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation.

1. Sreejit, G. et al. (2014) PLoS pathogens 10, e1004446. PMID: 25356553
Примечание For research use only.
Изображения
  • Anti-B2M Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-B2M polyclonal antibody at 1ug/mL on various samples.
    Lane 1: U937 cell lysate
    Lane 2: Raji cell lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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