Anti-Bacillus anthracis cya/EF/Edema factor Recombinant Antibody (A4) (JN040013)

Name: Anti-Bacillus anthracis cya/EF/Edema factor Recombinant Antibody (A4)
Brand: abinScience
SKU: JN040013
Price: 126 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN040013

Reactivité des espèces

Bacillus anthracis

Applications

ELISA, SPR

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a

ID de clone

Cible

Calmodulin-sensitive adenylate cyclase, 4.6.1.1, ATP pyrophosphate-lyase, Adenylyl cyclase, Anthrax edema toxin adenylate cyclase component, Edema factor, EF, cya, pXO1-122, BXA0141, GBAA_pXO1_0142, Bacillus anthracis

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P40136

RRID

Anti-Bacillus anthracis cya/EF/Edema factor Antibody (A4) (abinScience Cat# JN040013, RRID:AB_3729007)
_x005F_x005F_x000D_

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Calmodulin-sensitive adenylate cyclase (cya/EF) is a ~92 kDa protein. Edema factor (EF), which constitutes one of the three proteins composing the anthrax toxin, causes edema in the host. Acts as a calmodulin-dependent adenylyl cyclase by converting ATP to cAMP, leading to dramatic elevation of intracellular cAMP levels in the host, thereby causing edema. EF is not toxic by itself and only acts as an edema factor when associated with protective antigen (PA) to form the edema toxin (EdTx). Required for the survival of germinated spores within macrophages at the early stages of infection.

1. Drum, CL. et al. (2002) Nature 415, 396-402. PMID: 11807546

2. Xia, ZG. et al. (1990) The Journal of biological chemistry 265, 6517-20. PMID: 2108958

3. Leppla, SH. (1982) Proceedings of the National Academy of Sciences of the United States of America 79, 3162-6. PMID: 6285339

4. Kumar, P. et al. (2001) Infection and immunity 69, 6532-6. PMID: 11553601

5. Guidi-Rontani, C. et al. (2001) Molecular microbiology 42, 931-8. PMID: 11737637

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Bacillus anthracis cya/EF/Edema factor Recombinant Antibody (A4)

Reférences

Fiche technique

Anti-Bacillus anthracis cya/EF/Edema factor Recombinant Antibody (A4).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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