Anti-Bacillus anthracis cya/EF/Edema factor Recombinant Antibody (E3#) (JN040033)

Name: Anti-Bacillus anthracis cya/EF/Edema factor Recombinant Antibody (E3#)
Brand: abinScience
SKU: JN040033
Price: 191 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN040033

Reactividad de especies

Bacillus anthracis

Aplicaciones

ELISA, SPR

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a

ID de clon

E3#

Objetivo

Calmodulin-sensitive adenylate cyclase, 4.6.1.1, ATP pyrophosphate-lyase, Adenylyl cyclase, Anthrax edema toxin adenylate cyclase component, Edema factor, EF, cya, pXO1-122, BXA0141, GBAA_pXO1_0142, Bacillus anthracis

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P40136

RRID

Anti-Bacillus anthracis cya/EF/Edema factor Antibody (E3#) (abinScience Cat# JN040033, RRID:AB_3729009)
_x005F_x005F_x000D_

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Calmodulin-sensitive adenylate cyclase (cya/EF) is a ~92 kDa protein. Edema factor (EF), which constitutes one of the three proteins composing the anthrax toxin, causes edema in the host. Acts as a calmodulin-dependent adenylyl cyclase by converting ATP to cAMP, leading to dramatic elevation of intracellular cAMP levels in the host, thereby causing edema. EF is not toxic by itself and only acts as an edema factor when associated with protective antigen (PA) to form the edema toxin (EdTx). Required for the survival of germinated spores within macrophages at the early stages of infection.

1. Drum, CL. et al. (2002) Nature 415, 396-402. PMID: 11807546

2. Xia, ZG. et al. (1990) The Journal of biological chemistry 265, 6517-20. PMID: 2108958

3. Leppla, SH. (1982) Proceedings of the National Academy of Sciences of the United States of America 79, 3162-6. PMID: 6285339

4. Kumar, P. et al. (2001) Infection and immunity 69, 6532-6. PMID: 11553601

5. Guidi-Rontani, C. et al. (2001) Molecular microbiology 42, 931-8. PMID: 11737637

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Bacillus anthracis cya/EF/Edema factor Antibody (E3#)

Referencias

Datasheet

Anti-Bacillus anthracis cya/EF/Edema factor Recombinant Antibody (E3#).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo