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Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376) (JN898023)

Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376)
Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376)
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  • 100ug
  • 1mg
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  • SDS

Aperçu
Numéro de catalogueJN898023
Reactivité des espècesBacillus anthracis
ApplicationsELISA, WB, SPR
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de cloneSAA1376
Cible Protective antigen, PA, Anthrax toxins translocating protein, PA-83, PA83, Protective antigen PA-20, PA-20, PA20, Protective antigen PA-63, PA-63, PA63, pagA, pag, pXO1-110, BXA0164, GBAA_pXO1_0164
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P13423
RRID

Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (SAA1376) (abinScience Cat# JN898023, RRID:AB_3726894)
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Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Protective antigen (PA/pagA) is a ~85 kDa protein. Protective antigen constitutes one of the three proteins composing the anthrax toxin; it mediates attachment to host cells and translocation of edema factor (EF) and lethal factor (LF) into the host cytoplasm. PA associated with LF forms the lethal toxin (LeTx) and causes death when injected; PA associated with EF forms the edema toxin (EdTx) and produces edema. PA induces immunity to infection with anthrax.

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1. Bradley, KA. et al. (2001) Nature 414, 225-9. PMID: 11700562
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2. Bradley, KA. et al. (2003) The Journal of biological chemistry 278, 49342-7. PMID: 14507921
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3. Santelli, E. et al. (2004) Nature 430, 905-8. PMID: 15243628
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4. Lacy, DB. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 13147-51. PMID: 15326297
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5. Singh, Y. et al. (1991) The Journal of biological chemistry 266, 15493-7. PMID: 1651334
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6. Mock, M. et al. (2001) Annual review of microbiology 55, 647-71. PMID: 11544370
Note For research use only.
Images
  • Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376)

    Bioactivity

    Detects Bacillus anthracis PA/pagA/PA-83 in indirect ELISAs.

  • Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376)

    SDS-PAGE

    SDS-PAGE for Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (SAA1376).

  • Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (SAA1376)

    Western blot

    Western blot analysis was performed using anti-Bacillus anthracis PA/pagA/PA-83 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant Bacillus anthracis PA/pagA/PA-83 (Catalog No: JN898022)
    Lane 2: negative control

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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