Anti-Bacillus cereus gajA Polyclonal Antibody (JN922014)

Name: Anti-Bacillus cereus gajA Polyclonal Antibody
Brand: abinScience
SKU: JN922014
Price: 168 USD
Availability: InStock

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Especificación:

50ug
100ug

Número:

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Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN922014

Descripción

Anti-Bacillus cereus gajA Polyclonal Antibody (JN922014) is a rabbit polyclonal antibody detecting gajA in ELISA, IHC, WB. Suitable for Bacillus cereus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Bacillus cereus

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Bacillus cereus gajA (Gly258-Gly578).

Objetivo

Endonuclease GajA, 3.1.-.-, Gabija protein GajA, Nicking endonuclease GajA, gajA, IIE_04982

Purificación

Purified by antigen affinity column.

Número de acceso

J8H9C1

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Endonuclease GajA is a ~66 kDa protein. Component of antiviral defense system Gabija type I, composed of GajA and GajB. Endonuclease that nicks double-stranded DNA within the sequence 5'-TNNNCGGGNNA-3' in the absence of nucleotides (NTP, dNTP and NDPs), cleaving after C-1. Has no detected ATPase activity. Expression of Gabija type I in B.subtilis (strain BEST7003) confers resistance to phages phi105, phi29, rho14, SpBeta and SBSphiC. Expression of Gabija type I in E.coli B (strain ATCC 11303) confers resistance to phage T7.

1. Doron, S. et al. (2018) Science (New York, N.Y.) 359. PMID: 29371424

2. Cheng, R. et al. (2021) Nucleic acids research 49, 5216-5229. PMID: 33885789

3. Antine, SP. et al. (2024) Nature 625, 360-365. PMID: 37992757

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Bacillus cereus gajA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo