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Anti-Bacillus licheniformis BliGO/thiO Polyclonal Antibody (JN872014)

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Aperçu
Numéro de catalogueJN872014
Description
Anti-Bacillus licheniformis BliGO/thiO Polyclonal Antibody (JN872014) is a rabbit polyclonal antibody detecting BliGO in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesBacillus licheniformis
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Bacillus licheniformis BliGO/thiO (Met1-Arg369).
Cible Glycine oxidase, GO, 1.4.3.19, BliGO, thiO
Purification Purified by antigen affinity column.
Numéro d'accès S5FMM4
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Glycine oxidase (BliGO/thiO) is a ~40 kDa protein. Catalyzes the FAD-dependent oxidative deamination of glycine, leading to glyoxylate, ammonia and hydrogen peroxide. Is also able to act on various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine), and thus may be involved in the degradation pathway that allows B.licheniformis J33-8 to grow with glyphosate as the sole source of carbon. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate.

1. Zhang, K. et al. (2016) Enzyme and microbial technology 85, 12-8. PMID: 26920475
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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