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Anti-Bacillus licheniformis BliGO/thiO Polyclonal Antibody (JN872014)

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概要
カタログ番号JN872014
説明
Anti-Bacillus licheniformis BliGO/thiO Polyclonal Antibody (JN872014) is a rabbit polyclonal antibody detecting BliGO in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Bacillus licheniformis
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Bacillus licheniformis BliGO/thiO (Met1-Arg369).
ターゲット Glycine oxidase, GO, 1.4.3.19, BliGO, thiO
精製 Purified by antigen affinity column.
アクセッション番号 S5FMM4
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Glycine oxidase (BliGO/thiO) is a ~40 kDa protein. Catalyzes the FAD-dependent oxidative deamination of glycine, leading to glyoxylate, ammonia and hydrogen peroxide. Is also able to act on various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine), and thus may be involved in the degradation pathway that allows B.licheniformis J33-8 to grow with glyphosate as the sole source of carbon. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate.

1. Zhang, K. et al. (2016) Enzyme and microbial technology 85, 12-8. PMID: 26920475
注意事項 For research use only.
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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